Journal: American journal of hematology

Article Title: Hepcidin-dependent and hepcidin-independent regulation of erythropoiesis in a mouse model of anemia of chronic inflammation

doi: 10.1002/ajh.23670

Figure Lengend Snippet: Iron accumulates in red pulp of Hepc1 (−/−) mice with sterile abscesses. Extramedullary hematopoiesis occurs in Hepc1 (−/−) mice with sterile abscesses, as evidenced by expanded red pulp between white pulp (WP) (C), when compared to untreated Hepc1 (−/−) controls (A). Spleen iron concentration is very low in both groups of Hepc1 (−/−) mice (see Table I). No Prussian blue-stained iron was visible in untreated Hepc1 (−/−) mice (B). However, Hepc1 (−/−) mice with sterile abscesses had sparse iron stain in the red pulp region (D). A field from one representative mouse of each group is pictured. [Color figure can be viewed in the online issue, which is available at wileyonlinelibrary.com.]

Article Snippet: Paraffin-embedded slides were prepared and sections were stained with hematoxylin-eosin (H&E) or Prussian blue stain for iron (Fe) with nuclear fast red counterstain by the Department of Molecular and Comparative Pathobiology Histology Core at the Johns Hopkins University School of Medicine (Baltimore, MD).

Techniques: Sterility, Concentration Assay, Staining